Large intestinal microbial composition and serum levels of certain pro-inflammatory molecules in patients with myocardial infarction in a long-term period
DOI: 10.32364/2587-6821-2025-9-5-3
M.S. Stepanov1, N.S. Karpunina1, G.N. Spasenkov2
1Academician E.A. Vagner Perm State Medical University, Perm, Russian Federation
2Perm Territory Clinical Cardiological Dispensary, Perm, Russian Federation
Aim: to evaluate large intestinal microbial composition and serum levels of certain pro-inflammatory molecules in patients with myocardial infarction (MI) in a long-term period.
Materials and Methods: 101 patients were examined; a Group 1 included 51 MI patients with type 1 ST segment elevation, and 50 patients who did not have cardiovascular disease were enrolled in a Group 2. Acute and long-term levels of interleukin (IL) 6, IL1β, IL18, C-reactive protein (CRP), tumor necrosis factor alpha (TNFα), monocyte chemotactic protein-1 (MCP-1), and fatty acid binding protein (FABP) were determined in serum and supernatants. The first stool sample collected since MI onset was tested for dysbiosis with a follow-up testing in 207 [158; 214] days.
Results: as for levels of all the studied signaling molecules, acute MI patients significantly differed from their healthy peers. In a long-term period, regardless of the remaining significant difference, IL6, IL1β, IL18, MCP-1 and TNFα levels were substantially lower than in controls. Absence of clinical manifestations of dysbiosis and presence of almost normal levels of bifido-, lactobacilli and enterococci were associated with broader spectrum and an increase (often a significant one) in a number of facultative opportunistic pathogenic bacteria. A proportion of patients with a normal level of bifidobacteria and lactobacilli decreased to 44% and 72%, respectively (p=0.001 and p=0.03), with time. A proportion of typical and hemolytic E. coli, as well as enterococci, increased significantly (44%, 84%, 100%; p=0.01, p=0.03 and p=0.00, respectively).
Conclusion: regular changes in serum levels of proinflammatory molecules were reported in a long-term period of MI. In the first six months after the acute event, microbial landscape associated with cardiotropic therapy is characterized by increased proportion of opportunistic pathogenic bacteria. Mean number of microorganisms is not informative for a comparative analysis of small groups, and tolerant associations between microbiota state and a course of a post-infarction period can't be detected.
Keywords: ST segment elevation myocardial infarction, large intestinal dysbiosis, interleukins, long-term period.
For citation: Stepanov M.S., Karpunina N.S., Spasenkov G.N. Large intestinal microbial composition and serum levels of certain pro-inflammatory molecules in patients with myocardial infarction in a long-term period. Russian Medical Inquiry. 2025;9(5):275–280 (in Russ.). DOI: 10.32364/2587-6821-2025-9-5-3
About the authors:
Maxim S. Stepanov — Post-Graduate Student at the Department of Hospital Therapy and Cardiology, Academician E.A. Vagner Perm State Medical University; 26, Petropavlovskaya str., Perm, 614990, Russian Federation.
Natalia S. Karpunina — Dr. Sc. (Med.), Assistant Professor, Professor of the Department of Hospital Therapy and Cardiology, Academician E.A. Vagner Perm State Medical University; 26, Petropavlovskaya str., Perm, 614990, Russian Federation; ORCID iD 0000-0003-3127-1797
Grigoryi N. Spasenkov — C. Sc. (Med.), Cardiologist at the Perm Territory Clinical Cardiological Dispensary, Chief Consultant Cardiologist at the Ministry of Health of the Perm Territory; 84, Sibirskaya st., Perm, 614002, Russian Federation; ORCID iD 0000-0003-1085-5814
Contact information: Natalia S. Karpunina, e-mail: karpuninapsma@mail.ru
Financial Disclosure: no authors have a financial or property interest in any material or method mentioned.
There is no conflict of interest.
Received 10.06.2025.
Revised 17.06.2025.
Accepted 19.06.2022. Валеева Л.Л., Щербинина А.Е., Авдеева К.С. и др. Роль кишечной микробиоты в развитии сердечно-сосудистых заболеваний. Методы диагностики и способы коррекции (обзор). Журнал медико-биологических исследований. 2024;12(4):534–547. DOI: 10.37482/2687-1491-Z218Valeeva L.L., Shcherbinina A.E., Avdeeva K.S. et al. The role of gut microbiota in the development of cardiovascular diseases. Methods of diagnostics and ways of correction (review). Journal of Medical and Biological Research. 2024;12(4):534–547 (in Russ.). DOI: 10.37482/2687-1491-Z218
3. Яфарова А.А., Дементьева Е.В., Злобовская О.А. и др. Кишечная микробиота на различных стадиях сердечно-сосудистого континуума. Кардиоваскулярная терапия и профилактика. 2023;22(12):3751. DOI: 10.15829/1728-8800-2023-3751Yafarova A.A., Dementyeva E.D., Zlobovskaya O.A. et al. Gut microbiota at different stages of cardiovascular disease continuum. Cardiovascular Therapy and Prevention. 2023;22(12):3751 (in Russ.). DOI: 10.15829/1728-8800-2023-3751
4. Hennekens C.H., Hollar D., Agatston A.S. Aspirin and Statins to Decrease Risks of Cardiovascular Disease — The Need for Wider Utilization. US Cardiology. 2004;1(1):43–44. DOI: 10.15420/usc.2004.1.1.43
5. Nendl A., Andersen G.O., Seljeflot I. et al. Intestinal fatty acid binding protein is associated with infarct size and cardiac function in acute heart failure following myocardial infarction. Open Heart. 2024;11:e002868. DOI: 10.1136/openhrt-2024-002868
This work is licensed under a Creative Commons «Attribution» 4.0 License.
